If you believe that any review contained on our site infringes upon your copyright, please email us. All submitted reviews become the licensed property of Sheet Music Plus and are subject to all laws pertaining thereto.If you have any suggestions or comments on the guidelines, please email us. We cannot post your review if it violates these guidelines.Avoid disclosing contact information (email addresses, phone numbers, etc.), or including URLs, time-sensitive material or alternative ordering information.Please do not use inappropriate language, including profanity, vulgarity, or obscenity. Be respectful of artists, readers, and your fellow reviewers.This includes independent works designated by their composers as a Canon. Feel free to recommend similar pieces if you liked this piece, or alternatives if you didn't. The list below includes all pages in the category ' Canons '.Are you a beginner who started playing last month? Do you usually like this style of music? Consider writing about your experience and musical tastes.Do you like the artist? Is the transcription accurate? Is it a good teaching tool? George quietly and painlessly left this world while asleep on Sunday, June 4, 2023. Explain exactly why you liked or disliked the product. George Winston The Official George Winston Site GEORGE WINSTON (1949 - 2023) We are deeply saddened to share the news that George Winston has passed on after a 10-year battle with cancer.You may not digitally distribute or print more copies than purchased for use (i.e., you may not print or digitally distribute individual copies to friends or students). You are only authorized to print the number of copies that you have purchased. PLEASE NOTE: All Interactive Downloads will have a watermark at the bottom of each page that will include your name, purchase date and number of copies purchased. Once you download your personalized sheet music, you can view and print it at home, school, or anywhere you want to make music, and you don’t have to be connected to the internet. Interactive features include: playback, tempo control, transposition, melody instrument selection, adjustable note size, and full-screen viewing. Interactive Downloads are dynamic sheet music files that can be viewed and altered directly in My Digital Library from any device. Published by Hal Leonard - Digital (HX.38249). Piano Solo, Piano and Keyboard - Interactive Download By George Winston.
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The company's reputation for innovation and high-quality timepieces has made it highly respected in the watch industry. Today, Seiko is still producing a wide range of wristwatches, both with quartz and mechanical movements. The Spring Drive used a mainspring to power the watch, but also had a quartz regulator to keep time accurately. Seiko continued to push the boundaries of watchmaking in 1999 with the release of the Spring Drive, a watch that combined mechanical and quartz technologies. This watch used the movement of the wearer's wrist to generate electricity, which powered the quartz movement. 6/5 Spicewood ,800 Near Mint Hamilton Khaki Navy Automatic GMT 6/5 Spicewood 0. In 1982, Seiko launched the Seiko Kinetic, a watch that combined quartz accuracy with a self-winding mechanism. 6/1 Lakewood Novelty Vintage Watches Over 50 Different. With water resistance up to 600 meters, it quickly became a favorite among professional divers. In 1975, Seiko released the Seiko 7549-7009, the world's first professional saturation diving watch. This watch was more accurate and reliable than traditional mechanical watches, and changed the watch industry forever. In 1969, Seiko made history by introducing the Seiko Quartz Astron, the world's first quartz In 1960, the company released its first diving watch, the Seiko 6217-8001, which was water-resistant up to 150 meters and featured a rotating bezel to measure elapsed time. Seiko has a long history of producing innovative and high-quality wristwatches. In 1924, Seikosha introduced the Seiko Marvel, its first wristwatch with a chronometer-grade movement. It featured a 12-ligne movement and was powered by a cylinder escapement. The Seiko 5 collection is world famous for its robust automatic watches and great value for money. Automatic Purple Classic Day Date Mens Watch. Seikosha's first wristwatch, the Laurel, was produced in 1913. Seiko 5: Sporty and Elegant Automatic Watches. Vintage Seiko 5 Automatic 7009-8081 Day-Date Blue Dial Mens Wristwatch 36mm. He began producing his own timepieces in 1892 under the name Seikosha, which translates to "House of Precision." The company was founded in 1881 by Kintaro Hattori, who started out with a watch and jewelry shop in Tokyo, Japan. Clock's ticking, folks.Seiko is a Japanese watch company that has been producing wristwatches for over a century. Scroll through below to see all the options worth ditching your phone for, and then make moves-fast. We've sorted through the riffraff on Amazon and pulled together 35 can't-miss, affordable, stylish watches for you right here. Finding those watches, though? Not so easy, not at all. Though the internet's everything store might not initially sound like the go-to source to indulge your horological impulses, it's chock full of affordable watches from great brands like Timex, Seiko, Swatch, Fossil, and many, many more. Where to find these pieces, you ask? Amazon, of course. They perform their core function, they look damn good doing it, and they are super well-priced. Gold-tone stainless steel case with a black silicone with a brown leather top band. Some of the best options are, day we say it, cheap! By which we mean, they don’t cost an arm and leg. Wrist Watch SS 17 Jewels 58. Of course, luxury styles abound-from elegant dress watches to cinematic divers, all of them great in their own right-but you don't need to spend so much moola on your everyday timepiece if you're not in the mood. Seiko 5 Mechanical Automatic Vintage Watches All Auction Buy It Now 1,052 Results 5 filters applied Movement Dial Color Model Vintage Band Material Condition Price Buying Format All Filters Vintage Seiko 5 Men's Retro Automatic 7009A-cal. There's something reassuring about gazing at your wrist to check if you’re on schedule without bathing your brain in blue light-or having 20 new messages popping up, one right after the other, distracting you from your course of action. Only 1 left 1970s 'Vintage Japan' 'SEIKO 5. An analogue one, preferably, because staring at yet another screens is too much. vintage Seiko 5 Automatic Mens Day Date Mens Wrist Watch Good Condition. Exporting a video from Final Cut Pro to Compressor works nearly the same as using Compressor as a standalone application, but you can skip the intermediate step of creating a QuickTime movie. You can also send a video to Compressor by exporting a Browser clip or sequence directly from Final Cut Pro to Compressor. Then follow the directions for exporting video from Compressor. Make sure the Make Movie Self-Contained box is unchecked and click Save. Choose Current Settings and include both Audio and Video. To create a QuickTime reference movie, select the sequence or clip in the Browser that you want to export and choose File > Export > QuickTime Movie.Ĭhoose a name and location for the file. However, since this file simply references other media stored on your computer, it will not work on other computers that don’t have all the required media. This is because the video refers to your sequence’s original media files when possible, instead of storing all of the media in the file itself. These can be created quicker and takes up less space than a standard QuickTime movie. Since this will only be a temporary intermediate file, we recommend creating a QuickTime reference movie. To use Compressor as a standalone application, you must first create a QuickTime movie file to submit to Compressor. Look at you go! Using Compressor as a standalone application After it’s done exporting, you’re good to upload it to Wistia. Then, click Next, and choose where you want this file to export to. Next, make sure the resolution is set to 1080p, and that the Video Codec is set to Better Quality and not Faster Encode. This will change the codec to an H.264 MP4 file. In the Export File menu, under Settings, change the format from Video & Audio to Computer. Once here, double click Export File in the list of options and close the window. If Export File isn’t in the Share list, you’ll have to add it as a destination. From there choose File > Share > Export File. :) Sharing From Final Cut Pro XĮxporting videos for Wistia from Final Cut Pro is pretty straightforward.įirst, select a clip or sequence in the Browser, or open a sequence in the Timeline. To make things even easier, we’ve also put together an awesome Wistia preset for you to download. sending from Final Cut Pro to Compressor.using Compressor as a standalone application, or. There are three ways to export video for use on Wistia from Final Cut Pro: If you’re looking for free video transcoding software, we recommend Handbrake. Looking for a guide to optimal export settings? You should also see our export settings page. Here’s our tips and tricks to using them - a guide to exporting from video editors I have also seen some people recommend opening the resulting file in quicktime and then re-exporting it is this what I should do?Īlso, note that I have not purchased compressed yet (which yields another option in the share menu), but I will if this will yield the best quality.We know just enough to be dangerous about most video export software. mov file to youTube sometimes is problematic (like youTube might cause the audio levels or synch to be off etc.although I didn't seem to experience this with the first video I put up using this method). 264 is the way to get the "rawest" file, but that uploading the resulting. I have read that share -> export media with a video codec of H. I'm finally done creating the first episode of our retro-gaming show and I just wanted to get some opinions on the best way for me to export the finished product to youTube if my goal is to have maximum quality (even if the file size is large and it takes a while to upload). What are some causes of an abnormally large (dilated or mydriatic) pupil? On the other hand, if the difference in pupil size is larger in bright light, then the larger pupil may be the abnormal one because it is not getting smaller (or constricting) like it should. If the difference in size between the pupils is larger in the dark, then the smaller pupil may not be reacting well and could be the abnormal one. One of the most important parts of the exam for anisocoria is figuring out which pupil is not normal. How Does the Doctor Know if the Big Pupil is ‘Too Big’ or the Small Pupil is ‘Too Small’? Based on the exam, the ophthalmologist may do extra tests with eyedrops, blood work or CT scan/MRI. The ophthalmologist will look at the size of the pupils and how they react to bright and dim light. A complete eye exam is performed by an ophthalmologist to check vision, eyelid position, how the eyes move, and the health of the front and back of the eyes (among other things). How does the doctor determine whether anisocoria is due to an underlying medical problem?Ĭertain signs, such as when the anisocoria was first noted, whether it is more noticeable in bright or dim light, and whether there was an event that happened in the past that could have caused it, will help the ophthalmologist understand the cause. For example if there is a one millimeter difference in the size of the pupils in bright light, there will be a one millimeter difference in size in dim light as well. In physiologic anisocoria, the difference in pupil size does not usually change under bright or dim light. Typically, with physiologic anisocoria, the difference in pupil size between the two eyes is small, about one millimeter or less. Anisocoria that is NOT caused by a medical problem is called physiologic anisocoria. The amount of anisocoria can be different from day-to-day and can even switch eyes. Up to 30% of people have anisocoria and it is normal for them. Anisocoria can be normal (physiologic), or it can be a sign of a medical problem. The term anisocoria means that the pupils are not the same size at the same time. Normally pupils are in the same size, with both eyes dilating or constricting together. Is it normal to have pupils of different sizes? In the center, the dark spot is a hole in the iris called the pupil. 1: The iris is the colored part of the eye. When in a dark room the pupil usually gets bigger (or dilates) to allow more light to enter the eye. When in a bright room or in sunlight, the pupil usually gets smaller (or constricts). The size of the pupil depends on how much the iris muscle is constricted (tightened). The empty hole in the middle, which allows light to enter the eye, is called the pupil. It is a round muscle, similar in shape to a donut. The colored part of the eye is called the iris. Compression of the anterior cerebral artery leading to contra-lateral leg weakness. Herniation of the cingulate gyrus under the falx cerebri. This can lead to significant effect on the respiratory and cardiac centres leading to irregular respiratory rate. Diabetes insipidus due to compression on the pituitary stalkĬerebellar tonsils herniate through the foramen magnum.Tonsillar hernation due to progression compression.Impaired upward gaze due to pressure on the superior collicus effecting upward gaze centres.The effects of a central tentorial herniation can cause the following: Note the corticospinal tracts decussate above the pyramid hence any weakness will be contra-lateral and ipsilateral weakness is a false localising sign. This is compression of the cerebral peduncles (white matter conducting the longer fibre tracts) Ipsilateral Hemiparesis this is due to compression of Kernohans notch.Contra-lateral homonymous hemianopia due to compression of the posterior cerebral artery.Compression of the 3rd nerve nucleus in the midbrain leading to 3rd nerve palsy.Depressed Level of Consciousness due to interrupation of the reticular activating formation governing consciousness.When a nurse comes and tells you hey doc this patient has blown a pupil here is why! It is a sign of significantly raised ICP.Įffects of a Lateral tentorial herniation (temporal lobe herniates through the tentorial notch occupying the midbrain) This diagram is explains everything beautifully. Papilloedema- the optic nerve runs within the optic sheath, raised ICP impairs venous outflow and results in swelling.Vomiting- occurrs acute rise in ICP, due to activation of the vomiting centre area postrema.Headaches- worse in the morning, aggravated by bending, stooping. 1: Loloho Long, Long Honeymoon Maggie and I both love the Loloho YouTube channel. This channel focuses mostly on communicating while on the road – Mobile internet solutions, antenna boosters, mobile and wifi topicsĪ couple new to Full-time RVing documenting their experiences. Now that you know the most basic things about boondocking, let’s move onto our favorite RV boondocking tips. Hit the Road with Sean and Kristy RV YouTube Channel no. Long Long Honeymoon – Chronicles of a Full-Time RV Couple Grab a bite from the fridge, flip on the tube, or take a hot shower in your own bathroom.even when youre miles from civilization. Many great campsite videos including many “Top 5” Videos and many videos on Florida Camping locations Starry Night Wilderness Boondocking Camping with RV Recreational Vehicle Camper Van Class C. I find this to be one of the best general RV Topic channels with stories ranging from Boondocking and camper updates to traveling and life living in your camper. Boondocking is camping without campground hookups, and seriously, who needs them Boondocking enables you to camp anywhere you can find a place to park. Very specific content to Solar Energy and Solar solutions on RVs and Campers. As romantic as that sounds, the hard truth is that the most rewarding boondocking adventures are usually the result of a little. Thats no electricity, no water, and no sewer hookups. Sure, you could simply hop in your rig and head for the nearest patch of public land. Boondocking, also known as dry camping, is RVing without hookups. In the mean time – Here are some of our favorite Youtube Vloggers who produce a wealth of information for RV Owners on a wide range of topics that we believe you will find useful. From power needs to learning from others, here are five boondocking lessons to help you camp for free like a pro. In the upcoming future we will begin creating and publishing our own videos. The traditional Macchiato is a layered coffee drink with a somewhat strong espresso flavor. Mochas celebrate the flavors of chocolate and coffee together, making them quite a bit sweeter and toning down the intense flavor of espresso. Macchiatos have a strong espresso flavor with the addition of creaminess from the milk. If you’re ordering in Perth though, you’ll most likely get a double shot of espresso with the glass filled with textured milk. What is long macchiato?Ī traditional long macchiato is a double shot of espresso with a dash of textured milk and most of the glass left empty. It’s also much smaller than other drinks, with a standard serving clocking in at just 1 1/4 ounces (37 ml). Is a macchiato stronger than coffee?īecause the macchiato is usually made using just a small amount of milk, it has a much stronger flavor than other coffee drinks. The layers are supposed to be sipped in order, so mixing them essentially ruins the drink’s design. Macchiato layers make the drink unique, so the ombre of white to dark brown is the ideal look of a macchiato. The answer is no, do not mix a macchiato. Your barista might ask if that’s for here or to go. You can simply order the drink by asking for a macchiato or an espresso macchiato. How do you order a macchiato? Most shops dedicated to serving specialty coffee will know what a macchiato is. If they don’t have any syrup in them, which traditional ones don’t, they’ll be less sweet unless sweetener is added in some way. Speaking of tradition, the sweetness of macchiatos depends partly on how traditionally they’re made. Technically the name Macchiato Freddo (“Marked Cold”) is used for the cold Macchiato while Macchiato Caldo (“Marked Hot”) is used for the hot Macchiato. While some people prefer cold milk when making their Macchiato, more typically it is steamed and frothed hot milk that is used. McDonald’s is a massive worldwide fast-food franchise, and the McCafe is the McDonald’s menu offering various types of cafe-style drinks such as espresso drinks, iced coffees, blended coffee drinks, and more. An employee of this company estimated that a single shot of espresso would cost around $1. A McDonald’s employee told us that it’s pretty easy to order an espresso shot at the fast food chain. There is no espresso shot on the McDonald’s menu, but the good news is that Americans can get one. Can you get a shot of espresso at mcdonalds? McDonald’s officials said in a release today that their new offerings are made with freshly-brewed espresso and U.S.-sourced whole or nonfat milk. How much does espresso cost at McDonalds?.How many shots of espresso are in a McDonalds macchiato?.How many shots of espresso are in a McDonalds latte?.Why does McDonalds coffee taste different?.Who has better coffee McDonalds or Starbucks?.Is McCafé coffee the same as Mcdonalds?.Can you get a shot of espresso at mcdonalds?.Do you already have a Moka pot and tazzine at home? If not, this could be a good purchase for your next trip to Italy! In case you’re wondering which ones to buy, we’ll give you a tip: Bialetti is the inventor of the moka and definitely the most popular Italian moka brand. Of course you should drink the Italian coffee in a small tazzina. Important: Do not confuse this Italian coffee with mocha, the Turkish coffee (in Italian Caffè turco)! Since it is common in Italy to prepare coffee at home with a moka pot, but to go to a bar for an espresso, the respective meaning of Caffè is derived from the context: at home this means the coffee prepared with the moka pot, outside the home this is the Caffè Espresso. This coffee is called Caffè Moka, but in Italy it is simply called Caffè, just like Caffè Espresso. The taste is slightly different and unlike a real espresso, it does not produce a stable crema. Just add some ground coffee and water, put it on the stove and after a few minutes your Italian coffee is ready!Ĭoffee made with a Moka is not an espresso, because not enough pressure can be generated. There is one thing that should not be missing in an Italian house: la moka! This is the pot for preparing Italian coffee at home. He immediately hits the jackpot on multiple slot machines. Johnny, a cocky young gambler, arrives in Sin City and heads to Kadie's place. After slitting the boy's throat, he considers his coat and realizes he cannot remember how he acquired it. He questions the leader about being called "Bernini Boy", and learns that it is the brand of coat he is wearing. With the assistance of the deadly residents lurking in the shadows, he dispatches the frat boys. He follows the two surviving frat boys into The Projects, the neighborhood where he grew up. They flee, and Marv follows them, stealing a police car on the way, which he crashes into their car, leading to his blackout and memory loss. When Marv intervenes, the leader of the frat boys shoots him in the arm, calling him " Bernini Boy", which Marv mishears as "Bernie". Stepping outside, he encounters four rich frat boys burning a homeless man alive. He retraces his steps, recalling that since it's Saturday, he watched Nancy Callahan dance at Kadie's Saloon. Marv regains consciousness on a highway overlooking The Projects, surrounded by several dead young men and a crashed police car, with no memory of how he got there. Unlike the first film, A Dame to Kill For was a box-office bomb, grossing $39.4 million against its $65 million production budget, and received mixed reviews from film critics. The film was released on August 22, 2014, on 2D, 3D and RealD 3D. Newcomers to the series include Josh Brolin, Joseph Gordon-Levitt, Eva Green, Dennis Haysbert, Ray Liotta, Christopher Lloyd, Jamie Chung, Jeremy Piven, Christopher Meloni, Stacy Keach, Lady Gaga, Alexa Vega, Julia Garner, and Juno Temple. The film stars an ensemble cast including returning cast members Mickey Rourke, Jessica Alba, Rosario Dawson, Jaime King, Powers Boothe (in his final film role), and Bruce Willis. Two original stories ("The Long Bad Night" and "Nancy's Last Dance") were created exclusively for the film written by Miller. One of the smaller plots of the film is based on the short story "Just Another Saturday Night", which is collected in Booze, Broads, & Bullets, the sixth book in the comic series. Directed by Robert Rodriguez and Frank Miller, the script is written by Miller and is primarily based on the second book in the Sin City series by Miller, A Dame to Kill For. And, because of the advances that Blu-ray brings to movies.Sin City: A Dame to Kill For (also known as Frank Miller's Sin City: A Dame to Kill For) is a 2014 American action crime anthology film and follow-up to the 2005 film Sin City. Seeing a movie at its best is what being entertained, and Blu-ray is all about. One huge benefit of movies being on Blu-ray, is that the format preserves and even enhances (via HD transfer) the picture and sound that the filmmaker intended. If you want the best at-home movie viewing experience with engrossing visuals and sound, Blu-ray is the way to go. Gone With The Wind - 75th Anniversary Edition View All Blu-ray movies are still a thing, and as any video aficionado will tell you, Blu-ray was made specifically with home entertainment in mind, revolutionizing watching movies in high definition. Indiana Jones: The Complete Adventures 9.25/10 6. Premium pick: Harry Potter: Complete 8-Film Collection 8.65/10 3. Editors choice: The Lord of the Rings: The Motion Picture Trilogy 8.75/10 2. Moosejaw reserves the right to remove any user from the program and zero out their MJ$ balance if Moosejaw determines that the user is abusing the program by buying for resale, attempting to game the program or any other reason at Moosejaw's sole discretion.Duke Nukem 3D is a first-person shooter video game developed by 3D Realms. The intent of the program is to provide our loyal customers with a way to buy great gear for less. If you used the MJ$ and your balance is less than deduction, then your refund is reduced by the difference. If you return an item, we deduct the MJ$ earned on that item from your balance. You don't get earn MJ$ when you buy a gift card, but you do get MJ$ when you spend a gift card. Make sure to check us out on our social channels for other ways to win some extra MJ$! These special offers may have a cap on how many bonus MJ$ you earn during the promotion, but you will always earn the base Reward Dollars. So if we are running a 50% back special offer, and you buy a regular-priced item for $100.00, you will receive MJ$10.00 in base points, and a bonus of MJ$40. This is a transactional email that you will receive even if you are not subscribed to our marketing emails.Įvery so often, we'll have special offers where you can earn bonus MJ$, like an additional 10%, 20% or 40% back. We will also send you a monthly email that details your balance and other account activity. In stores, provide the email address attached to your account to learn your balance and apply to your purchase. Once signed in, you will see your available MJ$ and maximum that can be redeemed in the payment section of the checkout process. On, just sign-in on any page to see your available balance. MJ$ expire two years after earning and are redeemed on a first-in, first out basis (in other words, we always use your oldest MJ$ that are closest to expiration). You do not earn MJ$ for shipping or tax charges, only from the item value. "Discounted items" includes items on sale or discounted via coupon code or use of MJ$. Full-priced items earn 10% back while discounted items earn 2.5%. If you leave the rewards program, any unused MJ$ will be voided upon completion of your request.Īll items we sell are eligible with the exception of gift cards or other items specifically noted. You may choose to leave the Moosejaw Rewards program at any time by logging in and clicking on the “Opt out Rewards Program” link within the My Account Summary page, Email, calling 877-MOOSEJAW (87) or in-store with help from our employees. We really, really don't want to that to happen, so please opt-in or we'll feel terrible. Pre-existing program members that fail to opt-in prior to Jwill be removed from the program, and any unused MJ$ will be voided. Existing program members prior to that date must still formally opt-in and accept the terms and conditions to continue to retain, earn and redeem MJ$. The program opt-in requirement was added on August 6th, 2019. A customer opting-in to the Moosejaw Rewards program is a separate action from establishing a user profile as a result of making a purchase. Once opted-in to the program, MJ$ will earn and accrue for redemption as described below until the MJ$ expire or the user leaves the program.Īll information collected and used to facilitate the Moosejaw Rewards program is directly related to the program's ability to provide value to the customer. Users can opt-in to the program while checking out on or at one of our stores or at any time within 30 days of purchase to accrue the MJ$ earned on that purchase. The program is FREE to join and membership is ongoing until or unless the user requests to be removed or is removed due to abuse of the program. All users must accept these terms and conditions and create an account with Moosejaw to opt-in to the program and earn, accrue, retain and redeem Moosejaw Reward Dollars (MJ$). Mahalanabis M, Do J, Almuayad H et al (2010) An integrated disposable device for DNA extraction and helicase dependent amplification. doi: 10.1016/j.ab.2006.05.005Ĭhen Y, Seo TS (2011) PCR-free digital minisatellite tandem repeat genotyping. doi: 10.1021/ac3016533Īrcher MJ, Lin B, Wang Z, Stenger DA (2006) Magnetic bead-based solid phase for selective extraction of genomic DNA. Hwang K-Y, Kwon SH, Jung S-O et al (2012) Solid phase DNA extraction with a flexible bead-packed microfluidic device to detect methicillin-resistant Staphylococcus aureus in nasal swabs. Hwang K-Y, Kwon SH, Jung S-O et al (2011) Miniaturized bead-beating device to automate full DNA sample preparation processes for gram-positive bacteria. doi: 10.1016/j.bios.2008.09.015Ĭhou J, Wong J, Christodoulides N et al (2012) Porous bead-based diagnostic platforms: bridging the gaps in healthcare. Contains 211 references.įilipponi L, Sawant PD, Fulga F, Nicolau DV (2009) Microbeads on microposts: an inverted architecture for bead microarrays. We finally discuss the relevance and trends of the methods in terms of high-level multiplexed analysis and their potential in diagnosis and personalized medicine. We discuss selected trends such as microbead-coupled amplification, heterogeneous and homogenous DNA hybridization assays, real-time assays, melting curve analysis, and digital microbead assays. Specific sections cover chemical properties of microbeads, the coupling of targets onto solid surfaces, microbead probe systems (mainly oligonucleotide probes), microbead detection schemes (with subsections on suspension arrays, microfluidic devices, and immobilized microbeads), quantification of nucleic acids, PCR in solution and the detection of amplicons, and methods for solid-phase amplification. This review provides an overview on microbead-based platforms for biosensing with a main focus on nucleic acid detection (including amplification strategies and on selected probe systems using fluorescent labeling). The potential of microbead-based assays therefore is apparent, and commercial applications can be found in the detection and discrimination of single nucleotide polymorphism, of pathogens, and in trapping assays. Its advances include low costs, low workload, high speed and high-throughput automation. Microbead assays have been improved in the past decades and are now important tools in routine and point-of-care diagnostics as well as in life science. Nucleic acid-based methods include hybridization assays, solid-phase PCR, sequencing, and trapping assays. They also form the basis of various techniques for detection and quantification of nucleic acids and proteins. Microbead-based technologies represent elegant and versatile approaches for highly parallelized quantitative multiparameter assays. A coat of clear UV protection externally is recommended every 2-3 years. Simple maintenance contributes towards a longer lifespan of your log cabin. These include ongoing maintenance, location/weather conditions, and the materials used to build it. There will be many different factors that influence how long your log cabin lasts. With a wide range of log cabins for sale from just £58,210, it is easy to see why installing a Norwegian log cabin home on your land is an excellent long-term investment. Ideal to those wanting to relax and enjoy their home. Peace and quiet: Thanks to the same thermal mass that provides energy efficiency, you will be surprised with how quiet a log home is.They come with a full five-year warranty as standard. Long lasting: Our log homes are made for life and built to last.This results in a very strong, close-grained log. Strength unique to Scandinavian wood: Northern Scandinavia where the climate is cold means that trees grow very slowly.Sustainability: All our logs come from northern Scandinavia, where re-planting to ensure sustainability is a legal requirement.Any log home outside of the garden will require planning permission. An annexe in the garden would be supplied as a transportable log home, provided it is for a family member it will not require planning permission and in most cases it is exempt from Building regulations and VAT. Adds value to your home: A well-built and well-maintained log home is a great way of adding value to your property.You can expect any of these naturally insulated log cabins to stay cool in the summer and warm in the winter. This is due to the logs’ natural insulation and their natural temperature control properties. Energy efficient: Log homes are energy-efficient and excellent at retaining heat and conserving energy.Easy to install: Because they are easy to install, they cause little disruption on site.It will simply require a coat of clear UV protection every 2-3 years to protect it and maintain the lustre of the log. Internally, the log walls are maintenance free. Simple maintenance: A log home needs very little maintenance. |
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